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Identification of Constitutively Active AtMPK6 Mutants Using a Functional Screen in Saccharomyces cerevisiae

Bibliography:

Hudik E, Berriri S, Heribert Hirt​, Colcombet J., Identification of Constitutively Active AtMPK6 Mutants Using a Functional Screen in Saccharomyces cerevisiae. Methods Molecular Biology. 2014;1171:67-77. doi: 10.1007/978-1-4939-0922-3_6. PMID: 24908120​​​​

Authors:

Hudik E, Berriri S, Heribert Hirt, Colcombet J.

Keywords:

MAP Kinase , Functional genetic screen , Constitutive activity , Saccharomyces cerevisiae, In vivo cloning , Random mutagenesis

Year:

2014

Abstract:

​MAPK (Mitogen-Activated Protein Kinases) mutants which are active independently of phosphorylation by upstream MAPK Kinases (MAPKKs) help to clarify signal transduction processes through MAPK modules and provide a useful tool to understand MAPK roles in the cell. The identifi cation of such mutations is tricky. In this chapter, we provide a detailed protocol for their screening, taking advantage of a functional expression assay in yeast. 

ISSN:

1064-3745