Home > Publications > Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana.
Publications

Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana.

Bibliography:

Danquah A, de Zélicourt A, Boudsocq M, Neubauer J, Frei Dit Frey N, Leonhardt N, Pateyron S, Gwinner F, Tamby JP, Ortiz-Masia D, Marcote MJ, Heribert Hirt, Colc ​ombet J.​, Identification and characterization of an ABA-activated MAP kinase cascade in Arabidopsis thaliana. The Plant Journal. 2015 Feb 27. doi: 10.1111/tpj.12808​

Authors:

Danquah A, de Zélicourt A, Boudsocq M, Neubauer J, Frei Dit Frey N, Leonhardt N, Pateyron S, Gwinner F, Tamby JP, Ortiz-Masia D, Marcote MJ, Heribert Hirt, Colc​ombet J.​

Keywords:

ABA , Arabidopsis thaliana, MAP3K18, MAPK module, MKK3, signaling pathway

Year:

2015

Abstract:

​Abscisic acid (ABA) is a major phytohormone involved in important stress-related and developmental plant processes. Recent phosphoproteomic analyses revealed a large set of ABA-triggered phosphoproteins as putative MAPK targets, although the evidence for MAPKs involved in ABA signalling is still scarce. Here, we identified and reconstituted in vivo a complete ABA-activated MAPK cascade, composed of the MAP3Ks MAP3K17/18, the MAP2K MKK3 and the four C group MAPKs MPK1/2/7/14. In planta, we show that ABA activation of MPK7 is blocked in mkk3-1 and map3k17mapk3k18 plants. Coherently, both mutants exhibit hypersensitivity to ABA and altered expression of a set of ABA-dependent genes. A genetic analysis further reveals that this MAPK cascade is activated by the PYR/PYL/RCAR-SnRK2-PP2C ABA core signalling module through protein synthesis of the MAP3Ks, unveiling an atypical mechanism for MAPK activation in eukaryotes. Our work provides evidence for a role of an ABA-induced MAPK pathway in plant stress signalling.

ISSN:

1365-313X