Plant Science Seminar - Prof. Thorsten Nuernberger

3/17/2016 2:00 PM - 3/17/2016 3:30 PM


Patterns and receptors in plant immunity

Host pattern recognition receptor-mediated perception of microbe-associated molecular patterns (MAMP) is a prerequisite for the initiation of antimicrobial defenses in all multicellular organisms including plants. We report on the rapid identification of two novel pattern recognition receptors by exploiting the natural variation of PRRs within Arabidopsis ecotypes. A combination of GWAS and rad-seq genotyping was employed to identify novel pattern recognition receptors, RLP23 and RLP32, respectively, that contribute to immunity to microbial infection by sensing proteinaceous patterns from various microbes. Both receptors belong to the class of leucine rich-repeat (LRR) proteins lacking a cytoplasmic kinase domain, interact constitutively with and require SOBIR1 as a co-receptor, and recruit into the RLP/SOBIR1 complex another co-receptor, BAK1, in an ligand-dependent manner. Stable expression of RLP23 in tomato or potato conferred enhanced immunity to microbial infection, suggesting that interfamily transfer of plant pattern recognition receptors (PRRs) constitutes a generally applicable strategy for engineering durable immunity in crop plants. Negative regulation of PRR function, microbe-derived effector-mediated suppression of plant immunity and plant-mediated ligand processing for PRRs represent additional research fields in our lab that will be covered briefly.