Home > Publications > On the substrate
Publications

On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7

Bibliography:

Bruno M, Hofmann M, Vermathen M, Alder A, Beyer P, Al-Babili S​. On the substrate- and stereospecificity of the plant carotenoid cleavage dioxygenase 7.  FEBS Letters. 2014 May 2;588(9):1802-7. doi: 10.1016/j.febslet.2014.03.041. Epub 2014 Mar 28. PubMed PMID: 24685691.​

Authors:

Mark Bruno, Manuel Hofmann, Martina Vermathen, Adrian Alder, Peter Beyer, Salim Al-Babili

Keywords:

Apocarotenoid, Carlactone, Carotenoid, Carotenoid cleavage dioxygenase, Strigolactone

Year:

2014

Abstract:

Strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-β-carotene to form a supposedly 9-cis-configured β-apo-10′-carotenal. CCD8 converts this intermediate through a combination of yet undetermined reactions into the strigolactone-like compound carlactone. Here, we investigated the substrate and stereo-specificity of the Arabidopsis and pea CCD7 and determined the stereo-configuration of the β-apo-10′-carotenal intermediate by using Nuclear Magnetic Resonance Spectroscopy. Our data unequivocally demonstrate the 9-cis-configuration of the intermediate. Both CCD7s cleave different 9-cis-carotenoids, yielding hydroxylated 9-cis-apo-10′-carotenals that may lead to hydroxylated carlactones, but show highest affinity for 9-cis-β-carotene.

ISSN:

0036-8075