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Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors

Bibliography:

Dong Deng, Chuangye Yan, Xiaojing Pan, Magdy M. Mahfouz, Jiawei Wang, Jian-Kang Zhu, Yigong Shi, Nieng Yan. Structural Basis for Sequence-Specific Recognition of DNA by TAL Effectors. Science. DOI: 10.1126/science.1215670​

Authors:

Dong Deng, Chuangye Yan, Xiaojing Pan, Magdy M. Mahfouz, Jiawei Wang, Jian-Kang Zhu, Yigong Shi, Nieng Yan

Keywords:

N/A

Year:

2012

Abstract:

​TAL (transcription activator-like) effectors, secreted by phytopathogenic bacteria, recognize host DNA sequences through a central domain of tandem repeats. Each repeat comprises 33 to 35 conserved amino acids and targets a specific base pair by using two hypervariable residues [known as repeat variable diresidues (RVDs)] at positions 12 and 13. Here, we report the crystal structures of an 11.5-repeat TAL effector in both DNA-free and DNA-bound states. Each TAL repeat comprises two helices connected by a short RVD-containing loop. The 11.5 repeats form a right-handed, superhelical structure that tracks along the sense strand of DNA duplex, with RVDs contacting the major groove. The 12th residue stabilizes the RVD loop, whereas the 13th residue makes a base-specific contact. Understanding DNA recognition by TAL effectors may facilitate rational design of DNA-binding proteins with biotechnological applications.

ISSN:

1095-9203