Rashid Aman

Postdoctoral Fellows

Postdoctoral Fellow

Location:

3231-WS17

Research Interests

Rashid Aman is interested in exploring CRISPR/Cas technologies for virus interference and other RNA manipulations in plants. Cas9 has widely been exploited in developing various fruitful genome engineering technologies such as for virus interference and various genetic manipulations. However, the use of new CRISPR effectors for virus interference or other genetic manipulations remains challenging. We have successfully employed and screened different RNA targeting CRISPR/Cas13 effectors to determine the most effective one for virus interference in plants. Aman's research interests lie in understanding the use of CRISPR/Cas13 effectors for transcriptome engineering and other RNA manipulations in plants.

Selected Publications

  • LAMP-coupled CRISPR-Cas12a module for rapid and sensitive detection of plant DNA viruses, Ahmed Mahas, Norhan Hassan, Rashid Aman, Tin Marsic, Qiaochu Wang, Zahir Ali, and Magdy M. Mahfouz, Viruses, 2021, Mar.
  • Efficient, Rapid, and Sensitive Detection of Plant RNA Viruses With One-Pot RT-RPA–CRISPR/Cas12a Assay, Aman, Rashid, Ahmed Mahas, Tin Marsic, Norhan Hassan, and Magdy M. Mahfouz, Frontiers in Microbiology, 2020, 11: 3277.
  • iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2, Ali, Zahir, Rashid Aman, Ahmed Mahas, Gundra Sivakrishna Rao, Muhammad Tehseen, Tin Marsic, Rahul Salunke et al., Virus Research, 2020, 198129. (*Equal contribution)
  • Engineering herbicide resistance via prime editing in rice, Butt, Haroon, Gundra Sivakrishna Rao, Khalid Sedeek, Rashid Aman, Radwa Kamel, and Magdy Mahfouz, Plant Biotechnology Journal, 2020.
  • Nucleic Acid Detection Using CRISPR/Cas Bio-sensing TechnologiesRashid Aman, Ahmed Mahas, Mahas A, Mahfouz M, ACS Synthetic Biology, 2020, Mar 11.
  • CRISPR-Cas13d mediates robust RNA virus interference in plants, Mahas Ahmed*, Rashid Aman*, and Magdy Mahfouz, Genome Biology, 2019, 20.1: 1-16. (*Equal contribution)
  • Engineering RNA virus interference via the CRISPR/Cas13 machinery in ArabidopsisRashid Aman, Ahmed Mahas, Haroon Butt, Zahir Ali, Fatimah Aljedaani and Magdy Mahfouz, Viruses, 2018,Dec 19; 10:732.
  • A calcium/palmitoylation switch interfaces the signaling networks of stress response and transition to flowering, Hee Jin Park*, Rashid Aman*, Francisco Gamez-Arjona*, Irene Villalta, Raul Carranco, Marika Lindahl, Damaso Hornero-Mendez, Elena García, Ray A. Bressan, Sang Yeol Lee, Clara Sánchez-Rodriguez, Jose M Pardo, Woe-Yeon Kim, Francisco J. Quintero, and Dae-Jin Yun, 2021.  (*Equal contribution- Under revision)
  • RNA virus interference via CRISPR/Cas13a system in plantsRashid Aman, Zahir Ali, Haroon Butt, Ahmed Mahas, Fatimah Aljedaani, Muhammad Zuhaib Khan, Shouwei Ding and Magdy Mahfouz, Genome Biology, 2018, Jan 4; 19(1):1.
  • A keystone mutation in plant sodium transporter HKT1 enabling the evolution of salt toleranceAkhtar Ali, Natalia Raddatz, Rashid Aman, Songmi Kim, Hyeong Cheol Park, Dongwon Baek, Masood Jan, Dong-Ha Oh, Irfan Ullah Khan, Sang Yeol Lee, Ray A.Bressan, Keun Woo Lee, Jose M. Pardo, Albino Maggio, Hans J. Bohnert, Dae-Jin Yun, Plant Physiology, 2016, Jul; 171(3):2112-26.

Education

  • PhD, Environmental Biotechnology, Division of Applied Life Science, Gyeongsang National University, South Korea, 2015
  • MSc, Biotechnology, Swinburne University of Technology, Australia, 2009
  • BSc (Honors), Biotechnology, University of Peshawar, 2006

Professional Profile

  • 2017-present: Postdoctoral fellow, Genome Engineering Laboratory, Center for Desert Agriculture, KAUST, Saudi Arabia
  • 2016-2017: Visiting Scientist, Genome Engineering Laboratory, Center for Desert Agriculture, KAUST, Saudi Arabia
  • 2015-2016: Postdoctoral fellow, Cell Signaling Lab, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, South Korea

Awards

  • World Class University program, Gyeongsang National University, South Korea, 2009-2011 
  • Brain Korea Scholarship (BK-21), Gyeongsang National University, South Korea, 2012-2014 
  • Merit Scholarship, University of Peshawar, Khyber Pakhtunkhwa, Pakistan, 2002-2005 

Research Interests Keywords

synthetic biology plant tissue culture (tobacco and rice) virus based plant genome engineering